摘 要：
该研究从广西贺州信都三黄鸡样本中分离大肠杆菌，筛选致病性较强且耐药情况较严重的菌株，以此作为宿主菌分离与其相应的裂解性噬菌体。通过划线法分离出大肠杆菌菌株42株，用 PCR 分子法鉴定出34株阳性菌株，通过毒力基因 PCR 分子检测法，检测出5株致病性大肠杆菌。用K-B纸片法进行耐药性检测，检测出2株强耐药性的致病性大肠杆，以此作为宿主菌，分离纯化出2株噬菌体P1和P2。该研究为后续对噬菌体进行生物学特性以及噬菌体制剂的研究开发打下了基础。
关键词：噬菌体；大肠杆菌；PCR鉴定；药敏试验
Abstract:
This study isolated Escherichia coli from Xindu Sanhuang chicken samples, screened strains with strong pathogenicity and severe drug resistance, and used them as host bacteria to isolate corresponding lytic bacteriophages. 42 strains of Escherichia coli were isolated by streaking method, and 34 positive strains were identified by PCR molecular method. By using virulence gene PCR molecular detection method, 5 strains were identified as pathogenic Escherichia coli. Using K-B paper method to detect drug resistance of these 5 pathogenic Escherichia coli strains, 2 highly resistant pathogenic Escherichia coli strains were obtained. Using these two drug-resistant pathogenic Escherichia coli strains as host bacteria, two bacteriophages(P1 and P2) were isolated and purified. This study laid the foundation for further research on the biological characteristics of bacteriophages and bacteriophage preparations .
Keywords: Bacteriophage; Escherichia coli; PCR identification; Drug sensitivity test
--
