摘 要：
有研究表明茯砖茶有降脂减肥的功效，而茯砖茶中的优势菌种为金花菌。他汀类物质是较为全面的调脂药物，从微生物发酵液中检测他汀类物质的研究对象大多是曲霉，以金花菌为对象的研究较少，本论文以此为切入点。以从茯砖茶样分离得出的四种金花菌（标号为A、R、H和G9）为研究对象，对金花菌液体培养基中的碳源和氮源进行调节，并检测发酵液中他汀类物质的含量。结果表明：四种金花菌均能产洛伐他汀，不能产辛伐他汀，其中G9型金花菌产洛伐他汀能力最强，其发酵液中洛伐他汀的浓度最高可达11.624 mg/L，氮源比碳源更能影响金花菌生产洛伐他汀的产量。
关键词：金花菌；洛伐他汀；茯砖茶；培养基优化
Abstract: Studies have shown that Fuzhuan tea has the effect on reducing fat and weight, and the dominant bacteria in Fuzhuan tea is Eurotium cristatum. Statins are relatively comprehensive lipid-lowering drugs. There are few studies on Eurotium cristatum producing statins. In this study, four species of Eurotium cristatum (named A, R, H and G9) which isolated from Fuzhuan tea samples were used as research objects. Carbon and nitrogen sources in liquid culture medium are regulated, respectively, then those statins concentration in fermentation broth were detected by HPLC. The results show that all four species of Eurotium cristatum could produce lovastatin, could not produce simvastatin. The G9 type Eurotium cristatum has the strongest ability to produce lovastatin, and the highest concentration of lovastatin in fermentation broth is 11.624 mg/L. Meanwhile, comparing with carbon source, nitrogen source is mainly factor which affect lovastatin production in Eurotium cristatum fermentation broth.
Keywords: Eurotium cristatum; Lovastatin; Fuzhuan tea; Culture medium optimization
--
