摘 要：
为了提高金花菌和红曲霉原生质体产量，本实验以红曲霉和金花菌作为研究试材，研究菌龄、混合酶系统、酶解温度和酶解时间对金花菌和红曲霉原生质体制备的影响。结果表明，选取菌龄为5天的金花菌，加入0.5 mL 0.5 %纤维素酶和0.5 mL 0.5 %蜗牛酶在30 ℃条件下酶解2 h时，金花菌原生质体制备产量最佳，得到的原生质体产量为4.8×106个·mL-1；选取菌龄为4天的红曲霉，加入0.5 mL 0.5 %溶菌酶和0.5 mL 0.5 %纤维素酶在30 ℃条件下酶解2.5 h时，红曲霉原生质体制备产量最佳，得到的原生质体产量为4.3×106个·mL-1。通过设计单因素实验，确定获得高产红曲霉原生质体和高产金花菌原生质体的最适条件，为金花菌与红曲霉原生质体的融合奠定了基础。
关键词：金花菌；红曲霉；原生质体制备；条件优化
Abstract: In order to improve the protoplast production of Eurotium cristatum and Monascs, we did a series of experiments. In this experiment, Monascus and Eurotium cristatum were used as research materials to study the preparation ability of Monascus and Eurotium cristatum, such as bacterial age, mixed enzyme system, enzymolysis temperature and enzymolysis time. The results showed that Eurotium cristatum (cell age was 5 days) protoplast were prepared with 0.5 mL 0.5% cellulase and 0.5 mL 0.5% snailase at 30 ℃ for 2 hours, its yield was 4.8×106 per mL. Monascus(cell age was 4 days) protoplast were prepared with 0.5 mL 0.5% lysozyme and 0.5 mL 0.5% cellulase at 30 ℃ for 2.5 hours, its yield was 4.4×106/mL. By designing single-factor experiments, the optimal conditions for obtaining high-yield Monascus protoplasts and high-yield Eurotium cristatum protoplasts were determined, which laid the foundation for the Protoplast fusion of Eurotium cristatum and Monascus.
Keywords: Eurotium cristatum; Monascus; Protoplast preparation; Condition optimization
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