摘要：为了提高金花菌和红曲霉原生质体产量，本实验以红曲霉和金花菌作为研究试材，研究菌龄、混合酶系统、酶解温度和酶解时间对金花菌和红曲霉原生质体制备的影响。结果表明，选取菌龄为5天的金花菌，加入0.5 mL 0.5 %纤维素酶和0.5 mL 0.5 %蜗牛酶在30 ℃条件下酶解2 h时，金花菌原生质体制备产量最佳，得到的原生质体产量为4.8×106个·mL-1；选取菌龄为4天的红曲霉，加入0.5 mL 0.5 %溶菌酶【更多...】
SHOW FULL COLUMNS FROM `hl_config_system` [ RunTime:0.002217s ]
SELECT `value` FROM `hl_config_system` WHERE `type` = 'baseweb' AND `stype` = 'sys_view' AND `versions` = 1 LIMIT 1 [ RunTime:0.000556s ]
SELECT * FROM `hl_config_system` WHERE `type` = 'website' [ RunTime:0.003521s ]
SHOW FULL COLUMNS FROM `hl_foot_column` [ RunTime:0.001956s ]
SELECT `id`,`pid`,`title`,`url`,`type`,`link_to`,`link_way` FROM `hl_foot_column` WHERE `versions` = 1 AND `is_show` = 1 ORDER BY `sorted` DESC,`id` ASC [ RunTime:0.000706s ]
SELECT `value`,`stype` FROM `hl_config_system` WHERE `type` = 'baseweb' AND `versions` = 1 [ RunTime:0.000578s ]
SHOW FULL COLUMNS FROM `hl_journal_subject` [ RunTime:0.001536s ]
SELECT `id`,`subject_name` FROM `hl_journal_subject` WHERE `versions` = 1 ORDER BY `sort` DESC,`id` DESC [ RunTime:0.000331s ]
SHOW FULL COLUMNS FROM `hl_journal` [ RunTime:0.001910s ]
SELECT `jid`,`name` FROM `hl_journal` WHERE `versions` = 1 AND `isdelete` = 0 ORDER BY `is_recommend` DESC,`sort` DESC,`jid` DESC [ RunTime:0.000820s ]
SELECT * FROM `hl_config_system` WHERE `type` = 'baseweb' AND `stype` = 'default_journal' AND `versions` = 1 LIMIT 1 [ RunTime:0.000454s ]
SELECT * FROM `hl_journal` WHERE `shortname` = 'mpae' LIMIT 1 [ RunTime:0.001111s ]
SHOW FULL COLUMNS FROM `hl_paper` [ RunTime:0.002331s ]
SELECT COUNT(*) AS think_count FROM `hl_paper` `p` LEFT JOIN `hl_journal` `j` ON `p`.`jid`=`j`.`jid` WHERE `p`.`jid` = '19' AND `p`.`isdelete` = '0' AND `p`.`tags` <> '' AND `p`.`versions` = '1' LIMIT 1 [ RunTime:0.004186s ]
SELECT `p`.`id`,`p`.`title`,`p`.`files`,`p`.`addtime`,`p`.`status`,`p`.`isrec`,`p`.`authors`,`p`.`abstract`,`p`.`jid`,`p`.`quote`,`p`.`pid`,`p`.`release`,`p`.`intro`,j.name as jname,`p`.`open_access`,`p`.`list_authors`,`p`.`received`,`p`.`revised`,`p`.`accepted`,`p`.`published`,`p`.`cnum`,`p`.`dnum`,`p`.`orderno`,`p`.`cite`,`p`.`type`,`p`.`pages`,`p`.`star_pages`,`p`.`tags` FROM `hl_paper` `p` LEFT JOIN `hl_journal` `j` ON `p`.`jid`=`j`.`jid` WHERE `p`.`jid` = '19' AND `p`.`isdelete` = '0' AND `p`.`tags` <> '' AND `p`.`versions` = '1' ORDER BY `p`.`published` DESC,`p`.`sort` ASC LIMIT 0,15 [ RunTime:0.007886s ]
SHOW FULL COLUMNS FROM `hl_journal_period` [ RunTime:0.001907s ]
SELECT `name`,`pid`,`cyear`,`id` FROM `hl_journal_period` WHERE `isdelete` = 0 AND `versions` = 1 [ RunTime:0.000657s ]
SHOW FULL COLUMNS FROM `hl_paper_tag` [ RunTime:0.001637s ]
SELECT * FROM `hl_paper_tag` WHERE `id` = 69 ORDER BY `sort` DESC,`id` ASC [ RunTime:0.000457s ]
SELECT * FROM `hl_paper_tag` WHERE `id` = 69 ORDER BY `sort` DESC,`id` ASC [ RunTime:0.000436s ]
SELECT * FROM `hl_paper_tag` WHERE `id` = 69 ORDER BY `sort` DESC,`id` ASC [ RunTime:0.001083s ]
SELECT * FROM `hl_paper_tag` WHERE `id` = 70 ORDER BY `sort` DESC,`id` ASC [ RunTime:0.000297s ]
SELECT * FROM `hl_paper_tag` WHERE `id` = 70 ORDER BY `sort` DESC,`id` ASC [ RunTime:0.000563s ]
SELECT * FROM `hl_paper_tag` WHERE `id` = 70 ORDER BY `sort` DESC,`id` ASC [ RunTime:0.000313s ]
SELECT * FROM `hl_paper_tag` WHERE `id` = 70 ORDER BY `sort` DESC,`id` ASC [ RunTime:0.000252s ]
SELECT * FROM `hl_paper_tag` WHERE `id` IN (70,68) ORDER BY `sort` DESC,`id` ASC [ RunTime:0.000307s ]
SELECT * FROM `hl_paper_tag` WHERE `id` = 69 ORDER BY `sort` DESC,`id` ASC [ RunTime:0.000254s ]
SELECT * FROM `hl_paper_tag` WHERE `id` = 90 ORDER BY `sort` DESC,`id` ASC [ RunTime:0.000253s ]
SELECT * FROM `hl_paper_tag` WHERE `id` IN (70,90) ORDER BY `sort` DESC,`id` ASC [ RunTime:0.000408s ]
SELECT * FROM `hl_paper_tag` WHERE `id` IN (70,90) ORDER BY `sort` DESC,`id` ASC [ RunTime:0.000417s ]
SELECT * FROM `hl_paper_tag` WHERE `id` = 69 ORDER BY `sort` DESC,`id` ASC [ RunTime:0.000399s ]
SELECT `pid`,`name`,`type`,`cyear`,`id` FROM `hl_journal_period` WHERE `jid` = 19 AND `isdelete` = 0 AND `hide` = 0 ORDER BY `cyear` DESC,`type` ASC,`name` ASC,`ctime` ASC [ RunTime:0.001035s ]
SHOW FULL COLUMNS FROM `hl_journal_menu` [ RunTime:0.001596s ]
SELECT `id`,`column_title`,`column_content` FROM `hl_journal_menu` WHERE `jid` = 19 AND `is_show` = 1 [ RunTime:0.001336s ]
SHOW FULL COLUMNS FROM `hl_banner` [ RunTime:0.001486s ]
SELECT `id`,`img_url`,`url`,`group` FROM `hl_banner` WHERE `isshow` = 1 AND `type` = 2 AND `belongs_id` = 19 ORDER BY `sorted` ASC [ RunTime:0.000521s ]
