目的：探究枯草芽孢杆菌发酵条件优化，及其发发酵液各组分对水稻的促生长作用。方法:用单因素条件筛选和正交优化确定最佳培养基进行枯草芽孢杆菌的液体发酵。将枯草芽孢杆菌发酵液作用水稻，对比枯草芽孢杆菌发酵液各组分会对水稻生长的影响结果：枯草芽孢杆菌的最适的发酵培养基为糖蜜7.5 g/L、蛋白胨10 g/L、KH₂PO₄ 10 g/L，枯草芽孢杆菌发酵液小麦的生长都有促进作用，且枯草芽孢杆菌发酵液的不同成分及不同浓度对小麦的作用不同。从枯草芽孢杆菌发酵液提取的脂肽类物质对水稻的生长起到了促进作用。结论：枯草芽孢杆菌发酵液产的脂肽类物质对植物生长有一定的促生作用，但枯草芽孢杆菌发酵液对植物的生长起促进作用的其它物质，以及其作用的机理等有有待进一步的探究。

关键字：枯草芽孢杆菌；液体发酵；水稻

Abstract：

Objective: To explore the optimization of fermentation conditions for Bacillus subtilis and the promoting effects of various components in the fermentation broth on rice growth. Method: Single factor conditional screening and orthogonal optimization were used to determine the optimal culture medium for liquid fermentation of Bacillus subtilis. The fermentation broth of Bacillus subtilis was applied to rice, and the effects of various components on rice growth were compared. The results showed that the most suitable fermentation medium for Bacillus subtilis was 7.5 g/L of molasses, 10 g/L of peptone, and 10 g/L of KH₂PO₄. The Bacillus subtilis fermentation broth had a promoting effect on wheat growth, and different components and concentrations of Bacillus subtilis fermentation broth had different effects on wheat. The lipopeptides extracted from the fermentation broth of Bacillus subtilis have a promoting effect on the growth of rice. Conclusion: The lipopeptides produced by Bacillus subtilis fermentation broth have a certain promoting effect on plant growth, but further exploration is needed on other substances that promote plant growth in Bacillus subtilis fermentation broth, as well as their mechanisms of action.

Keywords: Gibberellins; Plant growth; Biosynthetic pathway; Agricultural and forestry production; Biomedicine

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