Objective: This study utilizes cellulase to extract flavonoids from Longhui Red Navel Orange Peel, and explores the optimal process conditions for flavonoid extraction. Method: A combination of single factor experiment and orthogonal experiment was used to investigate the effects of enzyme usage, ethanol concentration, solid-liquid ratio, extraction temperature, and extraction time on the yield of flavonoids from navel orange peel, and to explore the optimal extraction process. Result: This study found that the optimal process for extracting flavonoids from Longhui red navel orange peel using cellulase was as follows: enzyme dosage of 0.08 g, ethanol concentration of 70%, solid-liquid ratio of 1:25, extraction temperature of 50 ℃, extraction time of 120 minutes. The yield of flavonoids from navel orange peel was 1.52%, with an average recovery rate of 101.33%. Conclusion: This process has a good effect on the extraction of flavonoids from navel orange peel, and is simple, efficient, and stable. It can further provide theoretical reference for the development and utilization of navel orange peel resources and flavonoid extraction.
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